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mini protean tgx stain free protein gel  (Bio-Rad)


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    Bio-Rad mini protean tgx stain free protein gel
    Mini Protean Tgx Stain Free Protein Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+protean+tgx+stain+free+gel/MINI-PROTEAN+TGX/pmc13091216-163-38-44
    Average 99 stars, based on 13324 article reviews
    mini protean tgx stain free protein gel - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: 3 μg per sample was prepared for WB with 2X Laemmli loading buffer (BioRad, #1610737), and 1:10 beta-mercaptoethanol (BME) (Bio-Rad, #1610710). .. Samples and the biotinylated ladder (Cell Signaling, #7727) were run on a Mini-PROTEAN TGX Stain-Free gel (Bio-Rad, #4568124) and transferred to a nitrocellulose membrane (Immobilon-P, Millipore). .. Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)).

    Article Title: De novo variants in LDB1 are linked to distinct neurodevelopmental phenotypes determined by variant location and differing pathomechanisms
    Article Snippet: To assess protein stability, cells were treated with 25μM MG132 (Sigma Aldrich, St. Louis, MO, USA) 24 hours post transfection and incubated at 37°C for 4h prior to lysis. .. For SDS-Page, protein lysates were mixed with 4x SDS-Page sample buffer, separated on a 4-20% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad, Hercules, CA, USA) and subsequently blotted onto a nitrocellulose membrane using the semi dry blotting system (Bio-Rad). .. Membranes were incubated with the primary antibodies rabbit anti-FLAG (F7425, Sigma, 1:5,000) and rabbit anti-Histone H3 (4499, Cell Signaling Technology (CST), Danvers, MA, USA, 1:5,000), followed by incubation with goat anti-rabbit HRP conjugated secondary antibody (170-6515, Bio-Rad, 1:15,000).

    Article Title: Loss of nsp14-exonuclease activity impairs the replication, proofreading, fitness, and pathogenesis of SARS-CoV-2.
    Article Snippet: .. Protein was then concentrated with a PES concentrator (Thermo Sci., 88541), quantitated via protein assay kit (Thermo Sci., A53225), and visualized with a 4%–20% mini-Protean TGX Stain-free gel (BioRad) imaged with a BioRad Chemidoc MP imaging system. ..

    Article Title: Ageing promotes metastasis via activation of the integrated stress response
    Article Snippet: Proteins were isolated by using the 2× Laemmli Buffer (no. 1610737, BioRad) supplemented with β-mercaptoethanol (no. M3148, Sigma-Aldrich). .. Samples were subsequently heated at 95 °C for 10 min. Proteins were separated on 4–20% Mini-PROTEAN TGX Stain-Free gel (no. 4568096, BioRad) and then transferred onto a 0.2 μM nitrocellulose membrane (BioRad), incubated with specific primary antibodies listed in Supplementary Table . .. Protein bands were detected using Clarity Western ECL substrate (no. 1705061, BioRad) with the Amersham ImageQuant 800 western blot imaging systems (cytiva).

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: .. To assess protein purity, 3 μg of sample was prepared in 5 μL Laemmli buffer (10% (w/v) 2-mercaptoethanol, 4% (w/v) SDS, 20% (v/v) glycerol, 125mM Tris–HCl, and 0.004% (w/v) bromophenol blue) (Sigma Aldrich) and analyzed by SDS-polyacrylamide gel electrophoresis (PAGE). mCherry-containing proteins were only heated for 3min at 60°C to prevent cleavage [90], while other protein samples were denatured at 95°C for 3min and then applied to a 12-well Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad Laboratories). .. Images obtained from SDS-PAGE experiments were analyzed using the software ImageJ [91].

    Article Title: Domain-selective folding of the tandem β-propeller protein Coronin 7 (Coro7) by the chaperonin CCT/TRiC
    Article Snippet: .. The remaining 25 μl of sample was loaded onto a Mini-Protean TGX Stain-Free Gel, 4 to 20% (Bio-Rad) and run into the stacking gel. ..

    Article Title: AAV-mediated multiple gene therapy combining VEGFA-targeting miR-agshRNAs and PEDF for the suppression of choroidal neovascularization
    Article Snippet: .. Total protein concentration was determined, and 8 μg total protein and an equivalent volume of media were loaded onto a 12% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad) and run at 200 V for 30 min. .. Following electrophoresis, the gel was activated twice for 45 s, and proteins were transferred to a membrane (Trans-Blot Turbo Transfer Pack, 0.2 μm PVDF) (Bio-Rad) followed by blocking for 1 h at room temperature in 5% skim milk powder (VWR) (w/v) TBS-T, and incubation overnight at 4°C with monoclonal mouse anti-PEDF antibody (MAB1059, Merck Millipore), diluted 1:1000 in tris-buffered saline (TBS) (Thermo Fischer Scientific) with 0.1% Tween 20 (TBS-T).

    Article Title: ATP synthase is a promising target for identifying activated and non-activated adipose tissues.
    Article Snippet: Protein concentration of the supernatants was determined by Pierce BCA protein assay (Thermo Scientific). .. Equal amounts of protein (40 μg) were separated on Mini-PROTEAN TGX Stain-free gel (Bio-Rad, USA), transferred to Trans-blot turbo transfer pack membrane (Bio-Rad, USA) and probed for ATP5A1 (MA5-32609, Thermo Fisher, 1:2000 dilution) and β-actin (ab8227, abcam, 1:1000). .. Signal of the HRP-conjugated secondary antibody, goat anti-rabbit IgG (G21234, Invitrogen, 1:2500) was visualised by the Image Quant system (GE Healthcare Life Sciences).

    Membrane:

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: 3 μg per sample was prepared for WB with 2X Laemmli loading buffer (BioRad, #1610737), and 1:10 beta-mercaptoethanol (BME) (Bio-Rad, #1610710). .. Samples and the biotinylated ladder (Cell Signaling, #7727) were run on a Mini-PROTEAN TGX Stain-Free gel (Bio-Rad, #4568124) and transferred to a nitrocellulose membrane (Immobilon-P, Millipore). .. Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)).

    Article Title: De novo variants in LDB1 are linked to distinct neurodevelopmental phenotypes determined by variant location and differing pathomechanisms
    Article Snippet: To assess protein stability, cells were treated with 25μM MG132 (Sigma Aldrich, St. Louis, MO, USA) 24 hours post transfection and incubated at 37°C for 4h prior to lysis. .. For SDS-Page, protein lysates were mixed with 4x SDS-Page sample buffer, separated on a 4-20% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad, Hercules, CA, USA) and subsequently blotted onto a nitrocellulose membrane using the semi dry blotting system (Bio-Rad). .. Membranes were incubated with the primary antibodies rabbit anti-FLAG (F7425, Sigma, 1:5,000) and rabbit anti-Histone H3 (4499, Cell Signaling Technology (CST), Danvers, MA, USA, 1:5,000), followed by incubation with goat anti-rabbit HRP conjugated secondary antibody (170-6515, Bio-Rad, 1:15,000).

    Article Title: Ageing promotes metastasis via activation of the integrated stress response
    Article Snippet: Proteins were isolated by using the 2× Laemmli Buffer (no. 1610737, BioRad) supplemented with β-mercaptoethanol (no. M3148, Sigma-Aldrich). .. Samples were subsequently heated at 95 °C for 10 min. Proteins were separated on 4–20% Mini-PROTEAN TGX Stain-Free gel (no. 4568096, BioRad) and then transferred onto a 0.2 μM nitrocellulose membrane (BioRad), incubated with specific primary antibodies listed in Supplementary Table . .. Protein bands were detected using Clarity Western ECL substrate (no. 1705061, BioRad) with the Amersham ImageQuant 800 western blot imaging systems (cytiva).

    Article Title: ATP synthase is a promising target for identifying activated and non-activated adipose tissues.
    Article Snippet: Protein concentration of the supernatants was determined by Pierce BCA protein assay (Thermo Scientific). .. Equal amounts of protein (40 μg) were separated on Mini-PROTEAN TGX Stain-free gel (Bio-Rad, USA), transferred to Trans-blot turbo transfer pack membrane (Bio-Rad, USA) and probed for ATP5A1 (MA5-32609, Thermo Fisher, 1:2000 dilution) and β-actin (ab8227, abcam, 1:1000). .. Signal of the HRP-conjugated secondary antibody, goat anti-rabbit IgG (G21234, Invitrogen, 1:2500) was visualised by the Image Quant system (GE Healthcare Life Sciences).

    SDS Page:

    Article Title: De novo variants in LDB1 are linked to distinct neurodevelopmental phenotypes determined by variant location and differing pathomechanisms
    Article Snippet: To assess protein stability, cells were treated with 25μM MG132 (Sigma Aldrich, St. Louis, MO, USA) 24 hours post transfection and incubated at 37°C for 4h prior to lysis. .. For SDS-Page, protein lysates were mixed with 4x SDS-Page sample buffer, separated on a 4-20% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad, Hercules, CA, USA) and subsequently blotted onto a nitrocellulose membrane using the semi dry blotting system (Bio-Rad). .. Membranes were incubated with the primary antibodies rabbit anti-FLAG (F7425, Sigma, 1:5,000) and rabbit anti-Histone H3 (4499, Cell Signaling Technology (CST), Danvers, MA, USA, 1:5,000), followed by incubation with goat anti-rabbit HRP conjugated secondary antibody (170-6515, Bio-Rad, 1:15,000).

    Semi Dry Blot:

    Article Title: De novo variants in LDB1 are linked to distinct neurodevelopmental phenotypes determined by variant location and differing pathomechanisms
    Article Snippet: To assess protein stability, cells were treated with 25μM MG132 (Sigma Aldrich, St. Louis, MO, USA) 24 hours post transfection and incubated at 37°C for 4h prior to lysis. .. For SDS-Page, protein lysates were mixed with 4x SDS-Page sample buffer, separated on a 4-20% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad, Hercules, CA, USA) and subsequently blotted onto a nitrocellulose membrane using the semi dry blotting system (Bio-Rad). .. Membranes were incubated with the primary antibodies rabbit anti-FLAG (F7425, Sigma, 1:5,000) and rabbit anti-Histone H3 (4499, Cell Signaling Technology (CST), Danvers, MA, USA, 1:5,000), followed by incubation with goat anti-rabbit HRP conjugated secondary antibody (170-6515, Bio-Rad, 1:15,000).

    Imaging:

    Article Title: Loss of nsp14-exonuclease activity impairs the replication, proofreading, fitness, and pathogenesis of SARS-CoV-2.
    Article Snippet: .. Protein was then concentrated with a PES concentrator (Thermo Sci., 88541), quantitated via protein assay kit (Thermo Sci., A53225), and visualized with a 4%–20% mini-Protean TGX Stain-free gel (BioRad) imaged with a BioRad Chemidoc MP imaging system. ..

    Incubation:

    Article Title: Ageing promotes metastasis via activation of the integrated stress response
    Article Snippet: Proteins were isolated by using the 2× Laemmli Buffer (no. 1610737, BioRad) supplemented with β-mercaptoethanol (no. M3148, Sigma-Aldrich). .. Samples were subsequently heated at 95 °C for 10 min. Proteins were separated on 4–20% Mini-PROTEAN TGX Stain-Free gel (no. 4568096, BioRad) and then transferred onto a 0.2 μM nitrocellulose membrane (BioRad), incubated with specific primary antibodies listed in Supplementary Table . .. Protein bands were detected using Clarity Western ECL substrate (no. 1705061, BioRad) with the Amersham ImageQuant 800 western blot imaging systems (cytiva).

    Nucleic Acid Electrophoresis:

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: .. To assess protein purity, 3 μg of sample was prepared in 5 μL Laemmli buffer (10% (w/v) 2-mercaptoethanol, 4% (w/v) SDS, 20% (v/v) glycerol, 125mM Tris–HCl, and 0.004% (w/v) bromophenol blue) (Sigma Aldrich) and analyzed by SDS-polyacrylamide gel electrophoresis (PAGE). mCherry-containing proteins were only heated for 3min at 60°C to prevent cleavage [90], while other protein samples were denatured at 95°C for 3min and then applied to a 12-well Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad Laboratories). .. Images obtained from SDS-PAGE experiments were analyzed using the software ImageJ [91].

    Polyacrylamide Gel Electrophoresis:

    Article Title: Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex
    Article Snippet: .. To assess protein purity, 3 μg of sample was prepared in 5 μL Laemmli buffer (10% (w/v) 2-mercaptoethanol, 4% (w/v) SDS, 20% (v/v) glycerol, 125mM Tris–HCl, and 0.004% (w/v) bromophenol blue) (Sigma Aldrich) and analyzed by SDS-polyacrylamide gel electrophoresis (PAGE). mCherry-containing proteins were only heated for 3min at 60°C to prevent cleavage [90], while other protein samples were denatured at 95°C for 3min and then applied to a 12-well Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad Laboratories). .. Images obtained from SDS-PAGE experiments were analyzed using the software ImageJ [91].

    Protein Concentration:

    Article Title: AAV-mediated multiple gene therapy combining VEGFA-targeting miR-agshRNAs and PEDF for the suppression of choroidal neovascularization
    Article Snippet: .. Total protein concentration was determined, and 8 μg total protein and an equivalent volume of media were loaded onto a 12% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad) and run at 200 V for 30 min. .. Following electrophoresis, the gel was activated twice for 45 s, and proteins were transferred to a membrane (Trans-Blot Turbo Transfer Pack, 0.2 μm PVDF) (Bio-Rad) followed by blocking for 1 h at room temperature in 5% skim milk powder (VWR) (w/v) TBS-T, and incubation overnight at 4°C with monoclonal mouse anti-PEDF antibody (MAB1059, Merck Millipore), diluted 1:1000 in tris-buffered saline (TBS) (Thermo Fischer Scientific) with 0.1% Tween 20 (TBS-T).



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